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      A target-induced amperometic aptasensor for sensitive zearalenone detection by CS@AB-MWCNTs nanocomposite as enhancers

      Public Time: 2021-03-15 00:00:00
      Journal: Food Chemistry
      doi: 10.1016/j.foodchem.2020.128128
      Author: Zhaode Mu;Luyao Ma;Jie Wang;Jing Zhou;Yonghua Yuan;Lijuan Bai
      Summary: In this research, a novel signal-on aptasensor for highly sensitive detection of zearalenone (ZEN) was reported based on target-induced amplification strategy. Specifically, chitosan functionalized acetylene black and multi-walled carbon nanotubes (,) nanocomposite with large specific surface area and excellent conductivity was synthesized and served as the sensing platform. In addition, carboxylated graphene oxide-labeled ZEN binding aptamer (CGO-ZBA) would specifically recognized with ZEN to detach from the electrode, allowing the electrochemical signal of [Fe(CN),], increased more obviously. Under the optimal conditions, the proposed aptasensor exhibited exceptional detection performances for ZEN with a linear range from 10?fg?mL, to 1?ng?mL, and a low limit of detection of 3.64?fg?mL,. Given its great sensitivity, excellent selectivity, satisfactory stability and reproducibility, this method would provide a promising application for ZEN and other biomolecules by replacing the corresponding nucleic?acid?sequences.
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