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      Electrical unfolding of cytochrome

      Public Time: 2021-04-27 00:00:00
      Journal: PNAS
      doi: 10.1073/pnas.2016262118
      Author: Prabhat Tripathi,Abdelkrim Benabbas,Behzad Mehrafrooz,Hirohito Yamazaki,Aleksei Aksimentiev,Paul M. Champion,Meni Wanunu
      Summary: Can localized electric fields drive the complete unfolding of a protein molecule? Protein unfolding prior to its translocation through a nanopore constriction is an important step in protein transport across biological membranes and also an important step in nanopore-based protein sequencing. We studied here the electric-field–driven translocation behavior of a model protein (cyt c ) through nanopores of diameters ranging from 1.5 to 5.5 nm. These single-molecule measurements show that electric fields at the nanopore constriction can select both partially and fully unfolded protein conformations. Zero-field free energy gaps between these conformations, found using a simple thermodynamic model, are in remarkable agreement with previously reported studies of cyt c unfolding energetics. Datasets used to make figures have been deposited in Figshare ().
      Keyword tag: 
      nanopore biophysics ; cytochrome ; protein folding ; electric field unfolding ; solid-state nanopore
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